The SQLE Polyclonal Antibody (CAB2428) is specifically designed for research involving squalene epoxidase (SQLE), an enzyme involved in cholesterol biosynthesis and lipid metabolism. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the SQLE protein, researchers can effectively detect and analyze its expression in various cell types, making it an ideal tool for studies in lipid metabolism, cholesterol regulation, and related pathways.SQLE is a key enzyme in the mevalonate pathway, crucial for the production of sterols and isoprenoids that are essential for cell membrane structure and function. Dysregulation of SQLE activity has been implicated in various diseases, including cancer, cardiovascular diseases, and metabolic disorders.
By studying the role of SQLE in these conditions, researchers can gain valuable insights into disease mechanisms and potentially identify novel therapeutic targets for intervention.The SQLE Polyclonal Antibody offers researchers a reliable and effective tool for investigating the function and regulation of SQLE in diverse biological contexts. Its high specificity and sensitivity make it a valuable asset for advancing research in the fields of lipid biology, cholesterol metabolism, and the development of targeted therapies for diseases associated with dysregulated lipid pathways.
Product Name:
SQLE Rabbit Polyclonal Antibody
SKU:
CAB2428
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-400 of human SQLE (NP_003120.2).
Enables FAD binding activity and squalene monooxygenase activity. Involved in lipid droplet formation; regulation of cell population proliferation; and sterol biosynthetic process. Located in intracellular membrane-bounded organelle. Is integral component of membrane.
Purification Method:
Affinity purification
Gene ID:
6713
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using SQLE Rabbit pAb (CAB2428) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
