The SPTLC2 Polyclonal Antibody (CAB11716) is a high-quality research tool designed for studying the serine palmitoyltransferase long chain base subunit 2 (SPTLC2) protein. SPTLC2 is a key enzyme involved in sphingolipid biosynthesis, with implications in various cellular processes such as cell growth, differentiation, and apoptosis.This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for a wide range of applications including Western blotting, immunofluorescence, and immunohistochemistry. By specifically binding to SPTLC2, researchers can accurately detect and analyze the protein in different cell types and tissues, providing valuable insights into its biological functions.
Given the crucial role of SPTLC2 in sphingolipid metabolism and its implications in diseases such as cancer, neurodegenerative disorders, and metabolic conditions, this antibody serves as a valuable tool for investigating the molecular mechanisms underlying these pathologies. By elucidating the functions of SPTLC2, researchers can potentially identify novel therapeutic targets and develop treatments for these diseases.
Product Name:
SPTLC2 Rabbit Polyclonal Antibody
SKU:
CAB11716
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 423-562 of human SPTLC2 (NP_004854.1).
This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I.
Purification Method:
Affinity purification
Gene ID:
9517
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SPTLC2 Rabbit pAb (CAB11716) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
