The SPTA1 Polyclonal Antibody (CAB12355) is a valuable tool for researchers studying Spectrin, Alpha, Erythrocytic 1 (SPTA1), a critical component of the red blood cell membrane. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the SPTA1 protein, researchers can accurately detect and analyze the protein in various cell types, making it an ideal choice for studies in hematology and related fields.
SPTA1 is essential for maintaining the structural integrity of red blood cells and plays a crucial role in their function. Mutations in the SPTA1 gene have been implicated in various hematological disorders, making it a key target for research into conditions such as hereditary spherocytosis and hemolytic anemias. Understanding the role of SPTA1 in red blood cell biology is vital for developing new therapies and diagnostic tools for these diseases.
Product Name:
alpha 1 Spectrin Rabbit Polyclonal Antibody
SKU:
CAB12355
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 940-1160 of human alpha 1 Spectrin (NP_003117.2).
This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3.
Purification Method:
Affinity purification
Gene ID:
6708
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using alpha 1 Spectrin Rabbit pAb (CAB12355) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
