The SPPL2B Polyclonal Antibody (PACO60232) is a valuable tool for research involving SPPL2B, a protease enzyme involved in immune regulation and signal transduction. This antibody, produced in-house, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.SPPL2B, also known as signal peptide peptidase-like 2B, is a crucial player in the immune system, participating in the processing of immune response proteins and regulating immune cell activation.
Its role in immune function and signal transduction pathways makes it a promising target for studies in immunology, inflammation, and autoimmune diseases.By using the SPPL2B Polyclonal Antibody, researchers can effectively detect and analyze SPPL2B protein expression in different cell types, aiding in the understanding of its function and potential implications in disease pathology. This antibody is a valuable resource for scientists conducting research in the fields of immunology, inflammation, and autoimmune disorders.
Antibody Name:
SPPL2B Antibody (PACO60232)
Antibody SKU:
PACO60232
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Signal peptide peptidase-like 2B protein (492-592AA)
Immunofluorescence staining of A549 cells with PACO60232 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-α (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity. Catalyzes the intramembrane cleavage of the simian foamy virus processed leader peptide gp18 of the envelope glycoprotein gp130 dependently of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis.
Synonyms:
Signal peptide peptidase-like 2B (SPP-like 2B) (SPPL2b) (EC 3.4.23) (Intramembrane protease 4) (IMP-4) (Presenilin homologous protein 4) (PSH4) (Presenilin-like protein 1), SPPL2B, IMP4 KIAA1532 PSL1
UniProt Protein Function:
SPPL2B: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF- alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity. Belongs to the peptidase A22B family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Protease; EC 3.4.23.-; Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 19p13.3Cellular Component: actin cytoskeleton; centrosome; endosome membrane; lysosomal membrane; membrane; nucleoplasm; plasma membraneMolecular Function: aspartic endopeptidase activity, intramembrane cleaving; protein binding; protein homodimerization activityBiological Process: membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; membrane protein proteolysis; regulation of immune response; signal peptide processing
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the GXGD family of aspartic proteases. The GXGD proteases are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions. This enzyme localizes to endosomes, lysosomes, and the plasma membrane. It cleaves the transmembrane domain of tumor necrosis factor alpha to release the intracellular domain, which triggers cytokine expression in the innate and adaptive immunity pathways. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
