The SPP1 Polyclonal Antibody (CAB14062) is a valuable tool for researchers studying the SPP1 protein, also known as osteopontin, which is involved in various biological processes such as cell adhesion, migration, and immune response regulation. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.Osteopontin is a multifunctional protein that has been implicated in a range of diseases, including cancer, inflammation, and autoimmune disorders. By targeting SPP1 with this polyclonal antibody, researchers can accurately detect and analyze the protein in different cell types, aiding in the investigation of its role in various physiological and pathological processes.
With its ability to facilitate the study of osteopontin in immunology, cancer research, and other fields, the SPP1 Polyclonal Antibody (CAB14062) is a valuable asset for researchers seeking to deepen their understanding of the molecular mechanisms underlying disease progression and potential therapeutic targets.
Product Name:
Osteopontin Rabbit Polyclonal Antibody
SKU:
CAB14062
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 17-314 of human Osteopontin (NP_001035147.1).
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6696
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Osteopontin antibody (CAB14062) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
