The SPOP Polyclonal Antibody (CAB12106) is a valuable tool for researchers studying SPOP, a protein involved in regulating gene expression and cell growth. This antibody, produced in rabbits, has been tested for its reactivity with human samples and is suitable for use in Western blot applications. It specifically binds to the SPOP protein, allowing for accurate detection and analysis in various cell types.SPOP, also known as speckle-type POZ protein, is a key player in the regulation of the cell cycle and DNA repair processes. Dysregulation of SPOP has been implicated in various diseases, including cancer, making it an attractive target for therapeutic research.
Understanding the function of SPOP is essential for developing targeted treatments for cancer and other related conditions.Overall, the SPOP Polyclonal Antibody is a reliable tool for researchers aiming to investigate the role of SPOP in cellular processes and disease pathogenesis. Its specificity and sensitivity make it an ideal choice for studies focusing on molecular mechanisms underlying cancer development and progression.
Product Name:
SPOP Rabbit Polyclonal Antibody
SKU:
CAB12106
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 220-374 of human SPOP (NP_001007227.1).
This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.
Purification Method:
Affinity purification
Gene ID:
8405
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SPOP Rabbit pAb (CAB12106) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
