The Splicing Factor 1 Polyclonal Antibody (CAB6424) is a valuable tool for researchers studying splicing factor 1, a key player in the regulation of pre-mRNA splicing. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Splicing factor 1 is known to be involved in the splicing of precursor mRNA molecules, a crucial step in gene expression that can ultimately have a profound impact on protein diversity and cellular function. By targeting the splicing factor 1 protein, this antibody enables precise detection and analysis of splicing factor 1 in various cell types, making it an essential component in studies related to RNA processing and gene regulation.
The role of splicing factor 1 in the intricate process of mRNA splicing has implications for a wide range of research areas, including developmental biology, neurobiology, and cancer biology. By elucidating the function and mechanisms of splicing factor 1, researchers can gain valuable insights into how gene expression is controlled and potentially uncover new therapeutic targets for diseases associated with aberrant splicing.
Product Name:
SF1 Rabbit Polyclonal Antibody
SKU:
CAB6424
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SF1 (NP_973727.1).
This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
7536
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HepG2 cells, using SF1 antibody (CAB6424) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
