The SPICE1 Antibody (CAB7855) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the Spice1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal choice for research in molecular biology and cell signaling.Spice1, also known as a key regulator of cellular function, plays a crucial role in mediating signals within cells and coordinating various biological activities.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SPICE1 Antibody
SKU:
CAB7855
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SPICE, CCDC52, SPICE1
Positive Sample:
293T, HeLa, BT-474, MCF7
Cellular Localization:
Cytoplasm, Centriole, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle.
Calculated MW:
96kDa
Observed MW:
120kDa
Involved in metaphase plate congression; mitotic spindle assembly; and regulation of centriole replication. Located in centriole; centrosome; and spindle.
Purification Method
Affinity purification
Gene ID
152185
RRID
AB_2772367
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SPICE1 Rabbit pAb (CAB7855) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
