The SPHK2 Polyclonal Antibody (CAB6748) is a valuable tool for researchers studying the role of sphingosine kinase 2 (SPHK2) in various cellular processes. SPHK2 is an enzyme involved in the regulation of cell proliferation, survival, and migration, making it a key player in signaling pathways related to cancer and inflammation.This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting SPHK2 in human samples. It has been validated for use in Western blot applications, enabling researchers to accurately study the expression levels and localization of SPHK2 in different cell types.
By targeting SPHK2, researchers can gain insights into the mechanisms underlying cancer progression, immune responses, and inflammatory disorders. The ability to detect and analyze SPHK2 with this antibody makes it an essential tool for studies in oncology, immunology, and related fields. Understanding the functions of SPHK2 may lead to the development of targeted therapies for a range of diseases, from cancer to autoimmune conditions.
Product Name:
SPHK2 Rabbit Polyclonal Antibody
SKU:
CAB6748
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human SPHK2 (NP_001191088.1).
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
56848
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Raji cells, using SPHK2 antibody (CAB6748) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
