The SPHK1 Polyclonal Antibody (CAB0660) is a valuable tool for researchers studying the sphingosine kinase 1 (SPHK1) protein, which is involved in the regulation of various cellular processes, including cell proliferation, survival, and migration. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in Western blot applications.SPHK1 is a key enzyme in the sphingolipid pathway and plays a critical role in cell signaling, inflammation, and cancer progression. The SPHK1 Polyclonal Antibody binds specifically to SPHK1, allowing for the detection and analysis of the protein in different cell types.
This makes it an ideal choice for studying the role of SPHK1 in immunology, oncology, and other fields of research.Understanding the function and regulation of SPHK1 is important for developing targeted therapies for diseases such as cancer, inflammation, and autoimmune disorders. By using the SPHK1 Polyclonal Antibody, researchers can further investigate the mechanisms by which SPHK1 exerts its effects, leading to potential advancements in the treatment of these conditions.
Product Name:
SPHK1 Rabbit Polyclonal Antibody
SKU:
CAB0660
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-384 of human SPHK1 (NP_001136074.1).
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
8877
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SPHK1 antibody (CAB0660) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
