The SPHK1 Monoclonal Antibody is a valuable tool for researchers studying sphingosine kinase 1 (SPHK1), an important enzyme involved in sphingolipid metabolism and cell signaling pathways. This monoclonal antibody, developed specifically for use in research applications, has been validated for use in various experimental methods, including Western blotting and immunofluorescence.SPHK1 plays a critical role in regulating cell growth, survival, and migration, making it a potential target for therapeutic interventions in cancer and other diseases. By targeting SPHK1, researchers aim to better understand its role in cellular processes and its potential as a therapeutic target for various conditions.
This monoclonal antibody provides high specificity and sensitivity for detecting SPHK1 in various samples, enabling researchers to further explore the function and regulation of this enzyme in different cellular contexts. With its versatility and reliability, the SPHK1 Monoclonal Antibody is an invaluable tool for advancing research in the fields of cell biology, cancer biology, and drug discovery.
Product Name:
SPHK1 Monoclonal Antibody
SKU:
CAB22093
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human SPHK1 (NP_001136074.1).
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
8877
Clone Number:
ARC54487
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SPHK1 antibody (CAB22093) at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
