The SPHK1 Polyclonal Antibody (CAB0139) is a valuable tool for researchers studying SPHK1, a key enzyme involved in sphingosine-1-phosphate (S1P) signaling pathways. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the SPHK1 protein, this antibody enables the detection and analysis of SPHK1 in various cell types, making it an essential tool for studies in immunology, cancer research, and other fields where sphingolipid metabolism is involved.
SPHK1 plays a critical role in regulating cell survival, proliferation, and migration, making it a potential target for therapeutic interventions in diseases such as cancer, inflammatory disorders, and autoimmune diseases. Understanding the activity of SPHK1 and its downstream signaling pathways is essential for developing novel treatments that target sphingolipid metabolism. The SPHK1 Polyclonal Antibody is a reliable reagent for researchers investigating the role of SPHK1 in health and disease.
Product Name:
SPHK1 Rabbit Polyclonal Antibody
SKU:
CAB0139
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-384 of human SPHK1 (NP_001136074.1).
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
8877
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SPHK1 antibody (CAB0139) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
