The Spermine Oxidase Polyclonal Antibody (CAB11677) is a valuable tool for researchers studying spermine oxidase, an enzyme involved in the metabolism of polyamines. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to spermine oxidase, researchers can detect and analyze the enzyme in various cell types, making it ideal for investigations in biochemistry and cancer research.
Spermine oxidase plays a crucial role in polyamine catabolism, which is essential for cell growth, differentiation, and apoptosis. Dysregulation of spermine oxidase has been linked to various diseases, including cancer, making it an attractive target for therapeutic interventions. Understanding the function of this enzyme is key to developing treatments that target polyamine metabolism in disease contexts.
Product Name:
SMOX Rabbit Polyclonal Antibody
SKU:
CAB11677
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-544 of human SMOX (NP_787033.1).
Polyamines are ubiquitous polycationic alkylamines which include spermine, spermidine, putrescine, and agmatine. These molecules participate in a broad range of cellular functions which include cell cycle modulation, scavenging reactive oxygen species, and the control of gene expression. These molecules also play important roles in neurotransmission through their regulation of cell-surface receptor activity, involvement in intracellular signalling pathways, and their putative roles as neurotransmitters. This gene encodes an FAD-containing enzyme that catalyzes the oxidation of spermine to spermadine and secondarily produces hydrogen peroxide. Multiple transcript variants encoding different isoenzymes have been identified for this gene, some of which have failed to demonstrate significant oxidase activity on natural polyamine substrates. The characterized isoenzymes have distinctive biochemical characteristics and substrate specificities, suggesting the existence of additional levels of complexity in polyamine catabolism.
Purification Method:
Affinity purification
Gene ID:
54498
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SMOX Rabbit pAb (CAB11677) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
