The Speedy Protein A Polyclonal Antibody (CAB2153) is a highly specific antibody designed for research involving the Protein A tag, a commonly used affinity tag in protein purification and protein-protein interaction studies. This antibody, produced in rabbits, is validated for use in various applications including Western blot, immunoprecipitation, and immunofluorescence. It binds specifically to the Protein A tag, allowing for efficient and accurate detection and analysis of tagged proteins in different experimental settings.The Protein A tag is widely used in biotechnology and molecular biology for purification and detection of recombinant proteins.
The Speedy Protein A Polyclonal Antibody provides researchers with a reliable tool to study protein-protein interactions, protein expression levels, and localization of tagged proteins in cells.Overall, the Speedy Protein A Polyclonal Antibody (CAB2153) is a valuable reagent for researchers working in protein biology, biotechnology, and related fields, facilitating the study of Protein A-tagged proteins with high sensitivity and specificity.
Product Name:
SPDYA Rabbit Polyclonal Antibody
SKU:
CAB2153
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SPDYA (NP_877433.2).
Enables protein kinase activator activity and protein kinase binding activity. Involved in several processes, including G1/S transition of mitotic cell cycle; positive regulation of cell population proliferation; and positive regulation of protein kinase activity. Located in nucleoplasm.
Purification Method:
Affinity purification
Gene ID:
245711
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using SPDYA Rabbit pAb (CAB2153) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.
