Anti-SPECC1L Antibody (CAB15798)

Antibody Name:	Anti-SPECC1L Antibody
Antibody SKU:	CAB15798
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human SPECC1L (NP_056145.4).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	LO2, HeLa, A375

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human SPECC1L (NP_056145.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LHLR NELR DMRA QLGI NEDH SEGD EKSE KETI MAHQ PTDV ESTL LQLQ EQNT AIRE ELNQ LKNE NRML KDRL NALG FSLE QRLD NSEK LFGY QSLS PEIT PGNQ SDGG GTLT SSVE GSAP GSVE DLLS QDEN TLMD HQHS NSMD NLDS ECSE VYQP LTSS DDAL DAPS SSES EGIP SIER SRKG SSGN ASEV SVAC LTER IHQM EENQ HSTS EELQ ATLQ ELAD LQQI TQEL NSEN ERLG EEKV ILME SLCQ QSDK LEHF SRQI EYFR SLLD EHHI SYVI DEDV KSGR YMEL EQRY MDLA E
Gene ID:	23384
Uniprot:	Q69YQ0
Cellular Location:	Cell junction, Cytoplasm, cytoskeleton, gap junction, spindle
Calculated MW:	120kDa/124kDa
Observed MW:	168kDa

Synonyms:

SPECC1L, CYTSA, GBBB2, OBLFC1

Background:

This gene encodes a coiled-coil domain containing protein. The encoded protein may play a critical role in actin-cytoskeletal reorganization during facial morphogenesis. Mutations in this gene are a cause of oblique facial clefting-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A read-through transcript composed of SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and the downstream ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding.

UniProt Protein Function:	Function: Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration. Ref.10
UniProt Protein Details:	Subunit structure: May interact with both microtubules and actin cytoskeleton. Subcellular location: Cytoplasm cytoskeleton. Cytoplasm cytoskeleton spindle. Cell junction gap junction. Note: Colocalizes with acetylated alpha-tubulin, gamma-tubulin and F-actin. Also observed in a ring around gamma-tubulin containing centrioles possibly in the microtubule organizing center. Ref.10 Involvement in Disease: Facial clefting, oblique, 1 (OBLFC1) [MIM:600251]: A rare form of facial clefting. A facial cleft is any of the fissures between the embryonic prominences that normally unite to form the face.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10 Sequence similarities: Belongs to the cytospin-A family.Contains 1 CH (calponin-homology) domain.
NCBI Summary:	This gene encodes a coiled-coil domain containing protein. The encoded protein may play a critical role in actin-cytoskeletal reorganization during facial morphogenesis. Mutations in this gene are a cause of oblique facial clefting-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A read-through transcript composed of SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and the downstream ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]
UniProt Code:	Q69YQ0
NCBI GenInfo Identifier:	224451082
NCBI Gene ID:	23384
NCBI Accession:	NP_001138940.1
UniProt Related Accession:	Q69YQ0
Molecular Weight:
NCBI Full Name:	cytospin-A
NCBI Synonym Full Names:	sperm antigen with calponin homology and coiled-coil domains 1 like
NCBI Official Symbol:	SPECC1L
NCBI Official Synonym Symbols:	TBHS; CYTSA; GBBB2; OBLFC1
NCBI Protein Information:	cytospin-A
UniProt Protein Name:	Cytospin-A
UniProt Synonym Protein Names:	Renal carcinoma antigen NY-REN-22; Sperm antigen with calponin homology and coiled-coil domains 1-like
Protein Family:	Cytospin
UniProt Gene Name:	SPECC1L
UniProt Entry Name:	CYTSA_HUMAN

	Western blot analysis of extracts of various cell lines, using SPECC1L antibody (CAB15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded Human esophageal using SPECC1L Rabbit pAb (CAB15798) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)