Anti-SPDL1 Antibody (PACO20598)

Antibody Name:	SPDL1 Antibody (PACO20598)
Antibody SKU:	PACO20598
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:	Human
Immunogen:	Synthetic peptide of human SPDL1

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20598(SPDL1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
	The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using PACO20598(SPDL1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:

Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.

Synonyms:

spindle apparatus coiled-coil protein 1

UniProt Protein Function:	CCDC99: Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Belongs to the Spindly family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 5q35.1Cellular Component: cytosol; nucleus; outer kinetochore of condensed chromosome; spindle poleMolecular Function: enzyme binding; kinetochore binding; protein bindingBiological Process: establishment of mitotic spindle orientation; mitotic metaphase plate congression; sister chromatid cohesion
UniProt Protein Details:
NCBI Summary:	This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling. [provided by RefSeq, Jul 2016]
UniProt Code:	Q96EA4
NCBI GenInfo Identifier:	296452913
NCBI Gene ID:	54908
NCBI Accession:	Q96EA4.2
UniProt Secondary Accession:	Q96EA4,Q8TEC8, Q9HD44, Q9NX97, B4E393, C9JS47
UniProt Related Accession:	Q96EA4
Molecular Weight:	61,015 Da
NCBI Full Name:	Protein Spindly
NCBI Synonym Full Names:	spindle apparatus coiled-coil protein 1
NCBI Official Symbol:	SPDL1
NCBI Official Synonym Symbols:	CCDC99
NCBI Protein Information:	protein Spindly
UniProt Protein Name:	Protein Spindly
UniProt Synonym Protein Names:	Arsenite-related gene 1 protein; Coiled-coil domain-containing protein 99
Protein Family:	Protein Spindly
UniProt Gene Name:	SPDL1
UniProt Entry Name:	SPDLY_HUMAN

Antibodies

SPDL1 Antibody (PACO02989)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)