The SPDL1 Polyclonal Antibody (PAC020598) is a valuable tool for researchers studying the SPDL1 protein, which plays a key role in spindle dynamics and chromosome segregation during cell division. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the SPDL1 protein, this antibody enables precise detection and analysis in various cell types, making it an essential component for studies in cell biology and cancer research.SPDL1, also known as spindle apparatus coiled-coil protein 1, is essential for maintaining genomic stability and proper cell division. Dysregulation of SPDL1 has been implicated in various diseases, including cancer, where abnormal spindle dynamics can lead to chromosomal instability and tumor progression.
By uncovering the mechanisms by which SPDL1 functions, researchers can gain valuable insights into the pathogenesis of cancer and potentially identify new therapeutic targets for intervention.Overall, the SPDL1 Polyclonal Antibody is a reliable tool for investigating the role of the SPDL1 protein in cellular processes and disease development. Its specificity and sensitivity make it a valuable asset for researchers looking to advance our understanding of spindle dynamics and chromosome segregation in health and disease.
Antibody Name:
SPDL1 Antibody (PACO20598)
Antibody SKU:
PACO20598
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human SPDL1
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20598(SPDL1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using PACO20598(SPDL1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
Synonyms:
spindle apparatus coiled-coil protein 1
UniProt Protein Function:
CCDC99: Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Belongs to the Spindly family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 5q35.1Cellular Component: cytosol; nucleus; outer kinetochore of condensed chromosome; spindle poleMolecular Function: enzyme binding; kinetochore binding; protein bindingBiological Process: establishment of mitotic spindle orientation; mitotic metaphase plate congression; sister chromatid cohesion
UniProt Protein Details:
NCBI Summary:
This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling. [provided by RefSeq, Jul 2016]
