The SPC25 Polyclonal Antibody is a valuable tool for researchers studying SPC25, a key component of the NDC80 complex involved in chromosome segregation during mitosis. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting SPC25 in human samples, making it suitable for use in Western blot applications.
By targeting the SPC25 protein, researchers can gain insights into the mechanisms governing accurate chromosome segregation and cell division, with potential implications for cancer research and drug development. This antibody is an essential asset for studies focusing on cell cycle regulation and mitotic spindle assembly.
Product Name:
SPC25 Rabbit Polyclonal Antibody
SKU:
CAB10653
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 120-224 of human SPC25 (NP_065726.1).
This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity.
Purification Method:
Affinity purification
Gene ID:
57405
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SPC25 antibody (CAB10653) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
