Anti-SPATA16 Antibody (PACO15162)

Antibody Name:	SPATA16 Antibody (PACO15162)
Antibody SKU:	PACO15162
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:15-1:50
Species Reactivity:	Human, Mouse
Immunogen:	Fusion protein of human SPATA16

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO15162(SPATA16 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200).
	Gel: 12%SDS-PAGE, Lysate: 50 μg, Lane: Human liver cancer tissue, Primary antibody: PACO15162(SPATA16 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds.
	The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO15162(SPATA16 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200).

Background:

This gene encodes a testis-specific protein that belongs to the tetratricopeptide repeat-like superfamily. The encoded protein localizes to the Golgi apparatus and may play a role in spermatogenesis. The SPATA16 protein contains a region of homology to the mouse male enhanced antigen-2, a SCOP domain belonging to the tetratricopeptide repeat-like superfamily, 3 glycosylation sites, and several phosphorylation sites. The mouse and human proteins share 77% sequence identity. Northern blot analysis of multiple human tissues detected a strong signal in testis and weak expression in pancreas and kidney. Laser confocal microscopy localized a GFP-SPATA16 fusion protein specifically to the Golgi apparatus.

Synonyms:

Spermatogenesis associated 16

UniProt Protein Function:	SPATA16: Involved in the formation of sperm acrosome, which implicated its potential role in spermatogenesis and sperm-egg fusion. Defects in SPATA16 are the cause of spermatogenic failure type 6 (SPGF6); also known as round-headed spermatozoa. An infertility disorder caused by spermatogenesis defects. The most prominent feature is the malformation of the acrosome, which can be totally absent in most severe cases. Additional features are an abnormal nuclear shape and abnormal arrangement of the mitochondria of the spermatozoon. An autosomal recessive variation of SPATA16 has been shown to be responsible for the disease in a consanguineous family with members homozygous for the variation. Belongs to the SPATA16 family.Chromosomal Location of Human Ortholog: 3q26.31Disease: Spermatogenic Failure 6
UniProt Protein Details:
NCBI Summary:	This gene encodes a testis-specific protein that belongs to the tetratricopeptide repeat-like superfamily. The encoded protein localizes to the Golgi apparatus and may play a role in spermatogenesis. [provided by RefSeq, May 2010]
UniProt Code:	Q9BXB7
NCBI GenInfo Identifier:	190194384
NCBI Gene ID:	83893
NCBI Accession:	NP_114161.3
UniProt Secondary Accession:	Q9BXB7,Q0R0N4, Q0R0S0, Q0R0W2, Q0R129, Q0R131, Q0R140 Q0R1B8, Q0R1G5, Q0R1I2
UniProt Related Accession:	Q9BXB7
Molecular Weight:	65,263 Da
NCBI Full Name:	spermatogenesis-associated protein 16
NCBI Synonym Full Names:	spermatogenesis associated 16
NCBI Official Symbol:	SPATA16
NCBI Official Synonym Symbols:	SPGF6; NYD-SP12
NCBI Protein Information:	spermatogenesis-associated protein 16
UniProt Protein Name:	Spermatogenesis-associated protein 16
UniProt Synonym Protein Names:	Testis development protein NYD-SP12
Protein Family:	Spermatogenesis-associated protein
UniProt Gene Name:	SPATA16
UniProt Entry Name:	SPT16_HUMAN

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)