The SPAG8 Polyclonal Antibody (CAB8943) is a powerful tool for researchers studying SPAG8, a protein involved in sperm-associated antigen 8. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in various applications, including Western blotting. By binding to the SPAG8 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in reproductive biology and infertility research.SPAG8 is known for its role in sperm function and male fertility, making it a key target for investigations into reproductive health and infertility issues.
Researchers studying SPAG8 are interested in understanding its mechanisms and potential implications for fertility treatments and male contraception. By using the SPAG8 Polyclonal Antibody, scientists can gain valuable insights into the function and regulation of SPAG8 in reproductive processes, opening up new avenues for therapeutic development in this field.
Product Name:
SPAG8 Rabbit Polyclonal Antibody
SKU:
CAB8943
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human SPAG8 (NP_758516.1).
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.
Purification Method:
Affinity purification
Gene ID:
26206
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SPAG8 Rabbit pAb (CAB8943) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
