The SPAG4 Polyclonal Antibody (PACO18429) is a valuable tool for researchers interested in studying the SPAG4 protein, which plays a role in sperm motility and flagellar development. This antibody, produced in rabbits, has been shown to be highly specific for SPAG4 in human samples and is suitable for use in Western blot applications.SPAG4 is a key protein involved in the function of sperm tails, essential for sperm movement and fertilization. Research on SPAG4 is important in understanding male infertility and developing new treatments for reproductive issues.
By targeting SPAG4 with this antibody, researchers can investigate its role in sperm function and potentially uncover new insights into fertility and reproductive health.The SPAG4 Polyclonal Antibody is a reliable tool for researchers in the fields of reproductive biology, fertility, and developmental biology. Its specificity and sensitivity make it an ideal choice for studies aiming to elucidate the function of SPAG4 in various biological processes related to sperm motility and flagellar function.
Gel: 10%SDS-PAGE, Lysate: 50 μg, Lane: NIH/3T3 cells, Primary antibody: PACO18429(SPAG4 Antibody) at dilution 1/150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 minutes.
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using PACO18429(SPAG4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.
Synonyms:
sperm associated antigen 4
UniProt Protein Function:
Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.
UniProt Protein Details:
NCBI Summary:
The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
