SPAG4 Antibody (PACO18427)
- SKU:
- PACO18427
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
SPAG4 Antibody (PACO18427)
The SPAG4 Polyclonal Antibody (PAC018427) is a valuable tool for researchers studying SPAG4, a protein associated with sperm motility and male fertility. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for Western blotting applications. By targeting the SPAG4 protein, this antibody allows for the detection and analysis of SPAG4 expression in various cell types, making it an ideal choice for studies in reproductive biology and infertility research.SPAG4, also known as sperm-associated antigen 4, plays a crucial role in the movement and function of sperm cells, making it a key player in male reproductive health.
Understanding the mechanisms behind SPAG4 function is essential for unraveling the causes of male infertility and developing potential treatments to address fertility issues. By using the SPAG4 Polyclonal Antibody, researchers can delve deeper into the molecular pathways involving SPAG4 and its impact on sperm motility, ultimately advancing our knowledge of male reproductive biology.
| Antibody Name: | SPAG4 Antibody (PACO18427) |
| Antibody SKU: | PACO18427 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:1000-1:5000, WB:1:200-1:1000, IHC:1:25-1:100 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Synthetic peptide of human SPAG4 |
| Form: | Liquid |
| Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Purification Method: | Antigen affinity purification |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO18427(SPAG4 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200). |
 | Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: NIH/3T3 cells, Primary antibody: PACO18427(SPAG4 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute. |
 | The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO18427(SPAG4 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200). |
| Background: | The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. |
| Synonyms: | Sperm associated antigen 4 |
| UniProt Protein Function: | Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. |
| UniProt Protein Details: | |
| NCBI Summary: | The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
| UniProt Code: | Q9NPE6 |
| NCBI GenInfo Identifier: | 11037055 |
| NCBI Gene ID: | 6676 |
| NCBI Accession: | NP_003107.1 |
| UniProt Secondary Accession: | Q9NPE6,O43648 |
| UniProt Related Accession: | Q9NPE6 |
| Molecular Weight: | 48,165 Da |
| NCBI Full Name: | sperm-associated antigen 4 protein isoform 1 |
| NCBI Synonym Full Names: | sperm associated antigen 4 |
| NCBI Official Symbol: | SPAG4Â Â |
| NCBI Official Synonym Symbols: | SUN4; CT127Â Â |
| NCBI Protein Information: | sperm-associated antigen 4 protein |
| UniProt Protein Name: | Sperm-associated antigen 4 protein |
| UniProt Synonym Protein Names: | Outer dense fiber-associated protein SPAG4; SUN domain-containing protein 4 |
| Protein Family: | Sperm-associated antigen 4 protein |
| UniProt Gene Name: | SPAG4Â Â |
| UniProt Entry Name: |
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