SPAG4 Antibody (PACO18426)
- SKU:
- PACO18426
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
SPAG4 Antibody (PACO18426)
The SPAG4 Monoclonal Antibody (PAC018426) is a vital tool for researchers studying SPAG4, a protein involved in sperm motility and male infertility. This antibody, produced in mice, shows high specificity for human samples and has been validated for use in immunohistochemistry and flow cytometry applications. By binding to the SPAG4 protein, this antibody allows for precise detection and analysis of SPAG4 expression in various cell types, making it ideal for investigations in reproductive biology and infertility research.
SPAG4, also known as sperm-associated antigen 4, is essential for proper sperm function and is implicated in male infertility. Its role in sperm motility and fertilization makes it a crucial target for understanding infertility mechanisms and developing treatments for individuals struggling with infertility issues. Research on SPAG4 sheds light on the molecular pathways involved in reproductive health and may lead to advancements in fertility treatments and assisted reproductive technologies.
| Antibody Name: | SPAG4 Antibody (PACO18426) |
| Antibody SKU: | PACO18426 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide of human SPAG4 |
| Form: | Liquid |
| Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Purification Method: | Antigen affinity purification |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO18426(SPAG4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200). |
 | Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hela cells, K562 cells, Primary antibody: PACO18426(SPAG4 Antibody) at dilution 1/1100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds. |
 | The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO18426(SPAG4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200). |
| Background: | The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. |
| Synonyms: | Sperm associated antigen 4 |
| UniProt Protein Function: | Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. |
| UniProt Protein Details: | |
| NCBI Summary: | The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
| UniProt Code: | Q9NPE6 |
| NCBI GenInfo Identifier: | 11037055 |
| NCBI Gene ID: | 6676 |
| NCBI Accession: | NP_003107.1 |
| UniProt Secondary Accession: | Q9NPE6,O43648 |
| UniProt Related Accession: | Q9NPE6 |
| Molecular Weight: | 48,165 Da |
| NCBI Full Name: | sperm-associated antigen 4 protein isoform 1 |
| NCBI Synonym Full Names: | sperm associated antigen 4 |
| NCBI Official Symbol: | SPAG4Â Â |
| NCBI Official Synonym Symbols: | SUN4; CT127Â Â |
| NCBI Protein Information: | sperm-associated antigen 4 protein |
| UniProt Protein Name: | Sperm-associated antigen 4 protein |
| UniProt Synonym Protein Names: | Outer dense fiber-associated protein SPAG4; SUN domain-containing protein 4 |
| Protein Family: | Sperm-associated antigen 4 protein |
| UniProt Gene Name: | SPAG4Â Â |
| UniProt Entry Name: |
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