SP100 Polyclonal Antibody (CAB5851) is a high-quality antibody designed for research involving SP100, a protein associated with nuclear body formation and regulation of gene expression. Raised in rabbits, this antibody is highly reactive with human samples and validated for use in various applications, including Western blotting and immunofluorescence.SP100 is a nuclear antigen that plays a crucial role in regulating gene expression, DNA repair, and cellular processes. Its involvement in nuclear body formation also suggests a role in nuclear organization and function. This antibody binds specifically to the SP100 protein, allowing for detection and analysis in different cell types, making it a valuable tool for studies in molecular biology, epigenetics, and cell biology.
The significance of SP100 in nuclear function and gene regulation makes it an important target for research in various fields, including oncology and virology. Understanding the role of SP100 in cellular processes can provide insights into disease mechanisms and potential therapeutic targets. The SP100 Polyclonal Antibody (CAB5851) offers researchers a reliable tool for investigating the function of SP100 in different biological contexts.
Product Name:
SP100 Rabbit Polyclonal Antibody
SKU:
CAB5851
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 181-480 of human SP100 (NP_001193631.1).
This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein.
Purification Method:
Affinity purification
Gene ID:
6672
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SP100 antibody (CAB5851) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 30s.
