The SP (Substance P) ELISA Kit is designed for the quantitative detection of Substance P (SP) levels in various biological samples.
Substance P is a critical neuropeptide involved in a wide array of cellular activities, including neuronal communication, pain perception, and stress response. It plays a significant role in the body's neuroimmune systems by mediating inflammatory reactions and has been implicated in various inflammatory diseases, neuropathic pain, and stress-related disorders. Accurate measurement of Substance P is essential for understanding its involvement in disease mechanisms and for developing targeted therapeutic strategies. Assay Genie's SP (Substance P) ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's SP (Substance P) ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
SP (Substance P) ELISA Kit
SKU:
AEES00001
Target:
SP (Substance P)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SP. During the reaction, SP in the sample or standard competes with a fixed amount of SP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-101
94-107
90-102
Average (%)
96
100
96
1:4
Range (%)
97-108
86-94
90-98
Average (%)
104
91
94
1:8
Range (%)
87-95
91-103
98-112
Average (%)
91
99
105
1:16
Range (%)
86-97
96-108
91-103
Average (%)
91
103
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
93
EDTA plasma (n=5)
99-109
104
Cell culture media (n=5)
90-100
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
233.17
510.94
2017.45
206.77
515.17
2371.94
Standard deviation
12.87
23.61
104.91
12.18
23.85
125.24
C V (%)
5.52
4.62
5.2
5.89
4.63
5.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of SP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes SP in samples. No significant cross-reactivity or interference between SP and analogues was observed.
