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SOX8/9/17/18 Colorimetric Cell-Based ELISA

SKU:
CBCAB01045
Product Type:
ELISA Kit
ELISA Type:
Cell Based
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human
Mouse
Detection Method:
Colorimetric
$719
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Description

system_update_altDatasheetMSDS

SOX8/9/17/18 Colorimetric Cell-Based ELISA

The Human SOX8/9/17/18 Colorimetric Cell-Based ELISA Kit is a comprehensive tool for detecting levels of SOX8, SOX9, SOX17, and SOX18 in cell lysates, tissue homogenates, and other biological samples. This kit offers high sensitivity and specificity, ensuring precise and accurate results for your research needs.SOX proteins are key transcription factors involved in various biological processes, including embryonic development, cell fate determination, and stem cell maintenance. Dysregulation of SOX proteins has been implicated in numerous diseases, such as cancer, developmental disorders, and cardiovascular diseases, highlighting the importance of studying these proteins in research.

The Human SOX8/9/17/18 Colorimetric Cell-Based ELISA Kit provides a reliable and efficient method for quantifying SOX protein levels in your samples, facilitating insights into their roles in physiological and pathological processes. Whether you are studying developmental biology, regenerative medicine, or cancer research, this kit is an invaluable tool for advancing your understanding of SOX proteins and their functions.

Product Name:SOX8/9/17/18 Colorimetric Cell-Based ELISA
Product Code:CBCAB01045
ELISA Type:Cell-Based
Target:SOX8/9/17/18
Reactivity:Human, Mouse
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nmStorage/Stability:4°C/6 Months
Format:96-Well Microplate

The SOX8/9/17/18 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect SOX8/9/17/18 protein expression profile in cells. The kit can be used for measuring the relative amounts of SOX8/9/17/18 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on SOX8/9/17/18.

Qualitative determination of SOX8/9/17/18 concentration is achieved by an indirect ELISA format. In essence, SOX8/9/17/18 is captured by SOX8/9/17/18-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 30812/6662/64321/54345, UniProt ID: P57073/P48436/Q9H6I2/P35713, OMIM: 605923/114290/608160/610928/601618/607823, Unigene: Hs.243678/Hs.707993/Hs.98367/Hs.8619
Gene Symbol:SOX9
Sub Type:None
UniProt Protein Function:SOX8: May play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; nucleus

Molecular Function:DNA binding; protein heterodimerization activity; transcription factor binding

Biological Process: negative regulation of photoreceptor cell differentiation; transcription from RNA polymerase II promoter; fat cell differentiation; cell fate commitment; positive regulation of gliogenesis; in utero embryonic development; astrocyte fate commitment; positive regulation of transcription, DNA-dependent; male gonad development; cell maturation; signal transduction; Sertoli cell development; enteric nervous system development; peripheral nervous system development; osteoblast differentiation; retina development in camera-type eye; positive regulation of osteoblast proliferation; oligodendrocyte differentiation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of myoblast differentiation; neural crest cell migration; negative regulation of apoptosis

NCBI Summary:This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein may be involved in brain development and function. Haploinsufficiency for this protein may contribute to the mental retardation found in haemoglobin H-related mental retardation (ART-16 syndrome). [provided by RefSeq, Jul 2008]
UniProt Code:P57073
NCBI GenInfo Identifier:10720294
NCBI Gene ID:30812
NCBI Accession:P57073.1
UniProt Secondary Accession:P57073,P48436, Q9H6I2, P35713,
UniProt Related Accession:P57073
Molecular Weight:446
NCBI Full Name:Transcription factor SOX-8
NCBI Synonym Full Names:SRY (sex determining region Y)-box 8
NCBI Official Symbol:SOX8  
NCBI Protein Information:transcription factor SOX-8
UniProt Protein Name:Transcription factor SOX-8
Protein Family:Transcription factor
UniProt Gene Name:SOX8  
UniProt Entry Name:SOX8_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies (Anti-SOX8/9/17/18 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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