The SOST Polyclonal Antibody is a valuable tool for researchers studying the SOST protein, a key regulator of bone formation and density. This antibody, produced in rabbits, is highly sensitive and specific for detecting SOST in human samples, making it ideal for use in Western blot applications. By targeting SOST, researchers can investigate its role in bone metabolism and its potential as a therapeutic target for conditions such as osteoporosis.SOST, also known as sclerostin, acts as a negative regulator of bone formation by inhibiting the Wnt signaling pathway. Dysregulation of SOST has been implicated in conditions such as osteoporosis and other bone diseases, making it an important focus of research in the field of skeletal biology.
By studying SOST with this antibody, researchers can gain insights into its function and potentially uncover new strategies for treating bone-related disorders.Overall, the SOST Polyclonal Antibody is a valuable tool for researchers interested in investigating the role of SOST in bone metabolism and related disorders. Its high reactivity and specificity make it a reliable choice for Western blot analysis, enabling accurate and reproducible results in the study of skeletal biology.
Product Name:
SOST Polyclonal Antibody
SKU:
CAB24852
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-213 of human SOST (NP_079513.1).
Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
Purification Method:
Affinity purification
Gene ID:
50964
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SOST using SOST Rabbit pAb(CAB24852) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
