Anti-SOS2 Antibody (PACO63399)

Antibody Name:	SOS2 Antibody (PACO63399)
Antibody SKU:	PACO63399
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Son of sevenless homolog 2 protein (187-404AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: A549 whole cell lysate, SH-SY5Y whole cell lysate, HL60 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate. All lanes: SOS2 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 153, 150 kDa. Observed band size: 153 kDa.
	IHC image of PACO63399 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO63399 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Promotes the exchange of Ras-bound GDP by GTP.
Synonyms:	Son of sevenless homolog 2 (SOS-2), SOS2

UniProt Protein Function:	SOS2: a guanine nucleotide exchange factor that activates Ras by catalyzing the exchange of bound GDP for GTP. Interacts with GRB2.Protein type: Motility/polarity/chemotaxis; GEFs, Ras; GEFs; Adaptor/scaffoldChromosomal Location of Human Ortholog: 14q21Cellular Component: cytosolMolecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; DNA binding; protein heterodimerization activityBiological Process: positive regulation of small GTPase mediated signal transduction; axon guidance; regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q07890
NCBI GenInfo Identifier:	154689780
NCBI Gene ID:	6655
NCBI Accession:	NP_008870.2
UniProt Secondary Accession:	Q07890,Q15503, Q17RN1, D3DSB4
UniProt Related Accession:	Q07890
Molecular Weight:	152,979 Da
NCBI Full Name:	son of sevenless homolog 2
NCBI Synonym Full Names:	son of sevenless homolog 2 (Drosophila)
NCBI Official Symbol:	SOS2
NCBI Official Synonym Symbols:
NCBI Protein Information:	son of sevenless homolog 2; SOS-2; guanine nucleotide exchange factor; guanine nucleotide releasing factor
UniProt Protein Name:	Son of sevenless homolog 2
UniProt Synonym Protein Names:
Protein Family:	Son of sevenless
UniProt Gene Name:	SOS2
UniProt Entry Name:	SOS2_HUMAN

Antibodies

Secondary Antibody

Biological Role of GLP-1