Sodium (Na) Colorimetric Assay Kit

SKU:: MAES0212
UPC:
Product Type:: Assay
Instrument:: Microplate Reader
Detection Method:: Colorimetric
Sample Type:: Serum, Plasma, Tissue
Sensitivity:: 0.02 mmol/L
Range:: 0.02-20 mmol/L
Assay Time:: 15 min

€219

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Description

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Figure 1: Measuring Nav1.3 activity using ING-2 in engineered HEK Nav1.3 cells. A) Baseline subtracted, kinetic fluorescence data acquired using a Molecular Devices FlexStation® (Ex: 515 nm, Em: 545 nm, Cutoff: 530 nm) for all veratridine concentrations evaluated. Veratridine, an inhibitor of Nav channel inactivation, was added at 30 sec. B) Veratridine concentration response curve (CRC) in engineered HEK Nav1.3 cells. The estimated EC50 is 15 μM, and error bars represent standard deviation (n = 3). — Add to Cart

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Figure 2: Measuring Nav1.3 inhibition using ING-2 in engineered HEK Nav1.3 cells. Tetracaine concentration response curves (CRC) in HEK Nav 1.3 cells measured using ING-2. Cells were exposed to tetracaine, a local anesthetic known the block voltage-gated sodium channels, for 10 min. prior to the addition of veratridine (33.3 μM). Fluorescence (Ex: 515 nm, Em: 555 nm, Cutoff: 550 nm) was recorded at ~1 Hz on a Molecular Devices FlexStation® plate reader for 1.5 min. after the addition of veratridine for “Kinetic” data (pink). For “Endpoint” data (blue), a Cytation 5 was used to collect fluorescence (Ex: 525 nm Em: 545 nm) 30 minutes after the addition of veratridine. Error bars represent SEM (n = 3). — Add to Cart

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Detection Method:	Colorimetric method
Sensitivity:	0.02 mmol/L
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Inter-assay CV:	0.084
Intra-assay CV:	0.022

Storage:	2-8°C
Valid Period:	12 months

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Sodium (Na) Colorimetric Assay Kit

Description

Overview

Properties

Detection Principle

Additional Information

Product Name:	Sodium (Na) Colorimetric Assay Kit
Product Code:	MAES0212
Sample Type:	Serum, Plasma, Tissue
Assay Time:	15 min
Assay Type:	Quantitative
Detection Instrument:	Microplate reader (405 nm)

Sodium (Na) Colorimetric Assay Kit

Description

Overview

Properties

Detection Principle

Additional Information

Related Products

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