The SOAT1 Monoclonal Antibody is a powerful tool for researchers studying SOAT1, an enzyme involved in cholesterol metabolism and lipid droplet formation. This antibody, derived from mouse hybridomas, is highly specific to human SOAT1 and has been validated for use in various applications, including immunofluorescence and flow cytometry.SOAT1, also known as acyl-coenzyme A:cholesterol acyltransferase 1, plays a crucial role in the regulation of cholesterol levels in cells by esterifying excess cholesterol into cholesterol esters for storage. Dysregulation of SOAT1 has been implicated in various diseases, including atherosclerosis, Alzheimer's disease, and obesity.
Therefore, studying SOAT1 expression and activity is essential for understanding the mechanisms underlying these conditions and developing potential therapeutic interventions.Whether investigating lipid metabolism, cholesterol homeostasis, or disease progression, the SOAT1 Monoclonal Antibody provides researchers with a reliable tool for detecting and analyzing SOAT1 expression in cell and tissue samples. Its specificity and sensitivity make it a valuable asset for studies in biochemistry, cell biology, and metabolism research.
The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6646
Clone Number:
ARC59877
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using SOAT1 Rabbit mAb (CAB23284) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
