The U1A Rabbit Monoclonal Antibody (CAB3686) is a high-quality antibody specifically designed for research involving the U1A protein, an essential component of the spliceosome complex. This antibody is raised in rabbits and has been validated for use in various applications, including Western blot and immunofluorescence. The U1A protein is known for its role in pre-mRNA splicing and regulation of gene expression. It plays a crucial role in RNA processing and is essential for proper cellular function. The U1A Rabbit Monoclonal Antibody binds specifically to the U1A protein, enabling accurate detection and analysis in a wide range of cell types.
Research involving the U1A protein is important in understanding the mechanisms of gene regulation and splicing errors that can lead to various diseases, including cancer and genetic disorders. By using the U1A Rabbit Monoclonal Antibody, researchers can further explore the function and significance of the U1A protein in different biological contexts, leading to advancements in molecular biology and potential therapeutic interventions.
Product Name:
U1A/SNRPA Rabbit Monoclonal Antibody
SKU:
CAB3686
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U1A/SNRPA (P09012).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
U1A; Mud1; U1-A; U1A/SNRPA
Positive Sample:
HeLa,U-937,Rat brain,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
31kDa
Observed MW:
35kDa/
The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.
Purification Method:
Affinity purification
Gene ID:
6626
Clone Number:
ARC0823
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using U1A/SNRPA Rabbit mAb (CAB3686) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
