Anti-SNAP29 Antibody (CAB4290)

Antibody Name:	Anti-SNAP29 Antibody
Antibody SKU:	CAB4290
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNAP29 (NP_004773.1).

Application:	WB IHC IP
Recommended Dilution:	WB 1:1000 - 1:4000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HeLa, BxPC-3, HepG2, 293T, U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNAP29 (NP_004773.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSAY PKSY NPFD DDGE DEGA RPAP WRDA RDLP DGPD APAD RQQY LRQE VLRR AEAT AAST SRSL ALMY ESEK VGVA SSEE LARQ RGVL ERTE KMVD KMDQ DLKI SQKH INSI KSVF GGLV NYFK SKPV ETPP EQNG TLTS QPNN RLKE AIST SKEQ EAKY QASH PNLR KLDD TDPV PRGA GSAM STDA YPKN PHLR AYHQ KIDS NLDE LSMG LGRL KDIA LGMQ TEIE EQDD ILDR LTTK VDKL DVNI KSTE RKVR QL
Gene ID:	9342
Uniprot:	O95721
Cellular Location:	Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein, autophagosome membrane, cilium membrane
Calculated MW:	28kDa
Observed MW:	29kDa

Synonyms:

SNAP29, CEDNIK, SNAP-29

Background:

This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene.

UniProt Protein Function:	SNAP29: Involved in multiple membrane trafficking steps. Defects in SNAP29 are the cause of CEDNIK syndrome (CEDNIK). CEDNIK is a neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma. Belongs to the SNAP-25 family.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: SNARE complex; centrosome; neuron projection; cytoplasm; plasma membrane; synapse; cell junction Molecular Function:SNAP receptor activity; syntaxin binding Biological Process: synaptic vesicle fusion to presynaptic membrane; protein transport; exocytosis; autophagic vacuole fusion; vesicle targeting; synaptic vesicle priming Disease: Cerebral Dysgenesis, Neuropathy, Ichthyosis, And Palmoplantar Keratoderma Syndrome
NCBI Summary:	This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O95721
NCBI GenInfo Identifier:	6685982
NCBI Gene ID:	9342
NCBI Accession:	O95721.1
UniProt Related Accession:	O95721
Molecular Weight:	258
NCBI Full Name:	Synaptosomal-associated protein 29
NCBI Synonym Full Names:	synaptosomal-associated protein, 29kDa
NCBI Official Symbol:	SNAP29
NCBI Official Synonym Symbols:	CEDNIK; SNAP-29
NCBI Protein Information:	synaptosomal-associated protein 29; soluble 29 kDa NSF attachment protein; synaptosomal-associated protein, 29kD; vesicle-membrane fusion protein SNAP-29
UniProt Protein Name:	Synaptosomal-associated protein 29
UniProt Synonym Protein Names:	Soluble 29 kDa NSF attachment protein; Vesicle-membrane fusion protein SNAP-29
Protein Family:	SNAP25 homologous protein
UniProt Gene Name:	SNAP29
UniProt Entry Name:	SNP29_HUMAN

	Western blot analysis of extracts of various cell lines, using SNAP29 antibody (CAB4290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human liver damage using SNAP29 antibody (CAB4290) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using SNAP29 antibody (CAB4290) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SNAP29 antibody (CAB4290) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 3ug SNAP29 antibody (CAB4290). Western blot was performed from the immunoprecipitate using SNAP29 antibody (CAB4290) at a dilition of 1:1000.

Antibodies

Anti-SNAP29 Antibody (CAB19952)[KO Validated]

SNAP29 Antibody (PACO12358)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)