The Snai2 Polyclonal Antibody (CAB1057) is a valuable tool for researchers studying Snai2, a transcription factor crucial for the regulation of epithelial-to-mesenchymal transition (EMT) in both normal development and cancer progression. The antibody, produced through immunization of rabbits, demonstrates high specificity for human samples and is suitable for use in Western blot analysis.Snai2, also known as Slug, plays a pivotal role in promoting EMT, a process involved in embryogenesis, wound healing, and cancer metastasis. Its dysregulation has been implicated in the progression and aggressiveness of various cancers, making it a promising target for therapeutic intervention.
By targeting Snai2, researchers aim to better understand the mechanisms underlying EMT and its contribution to cancer dissemination.This polyclonal antibody enables the detection and quantification of Snai2 protein levels in different cell types, offering valuable insights into its functional role in cellular processes. Its application in cancer research, particularly in elucidating EMT dynamics and tumor progression, makes it a valuable asset for scientists seeking to unravel the complexities of cancer biology and identify potential therapeutic strategies.
Product Name:
Slug Rabbit Polyclonal Antibody
SKU:
CAB1057
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human Slug (NP_003059.1).
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.
Purification Method:
Affinity purification
Gene ID:
6591
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse spleen, using Slug Rabbit pAb (CAB1057) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
