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SMZ ( Sulfamethoxazole) ELISA Kit (FSES0016)

SKU:
FSES0016
Product Type:
ELISA Kit
Applications:
ELISA
Research Area:
Food Science
€499
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Description

Technical Manual

SMZ ( Sulfamethoxazole) ELISA Kit

Kit Name: SMZ ( Sulfamethoxazole) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Sulfamethoxazole (SMZ) in samples, such as muscle, milk, honey, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, SMZ in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-SMZ antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of SMZ. The concentration of SMZ in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.1 ppb (ng/mL)
Reaction Mode: 25°C, 45 min ˜15 min
Detection Limit: Muscle (method 1) ---0.1 ppb; Muscle (method 2) ---1 ppb, Honey---0.1 ppb, Serum, Urine, Egg---0.4 ppb; Milk---2 ppb; Feed---4 ppb
Cross Reactivity: Sulfamethoxazole---100%
Sample Recovery Rate: Muscle, Honey, Egg ---85%±25%, Serum, Urine, Milk, Feed---80%±25%
Kits Components:
Item Specifications
ELISAMicrotiter plate 96 wells
Standard Liquid 1 mL each (0ppb, 0.1ppb, 0.3ppb, 0.9ppb, 2.7ppb, 8.1ppb)
HRP Conjugate 5.5 mL
Antibody Working Solution 5.5 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
20 x Concentrated Wash Buffer 40 mL
2 x Reconstitution Buffer 50 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: Ethyl acetate, Concentrated HCl, N-hexane,Acetonitrile, Na2HPO4·12H2O, NaOH NaH2PO4·2H2O.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.

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