The SMYD4 Polyclonal Antibody (CAB2337) is a valuable tool for researchers studying SMYD4, a protein involved in epigenetic regulation and cellular signaling pathways. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal reagent for Western blot applications. By targeting the SMYD4 protein, researchers can accurately detect and analyze its expression in various cell types, facilitating investigations in fields such as epigenetics, cancer biology, and developmental biology.
SMYD4, a member of the SET and MYND domain-containing protein family, is known to play a role in histone methylation and gene transcription. Dysregulation of SMYD4 has been implicated in various diseases, including cancer and cardiovascular disorders, highlighting its importance as a potential therapeutic target. By utilizing the SMYD4 Polyclonal Antibody, researchers can further elucidate the functions of this protein and its impact on cellular processes, paving the way for potential therapeutic interventions in related pathologies.
Product Name:
SMYD4 Rabbit Polyclonal Antibody
SKU:
CAB2337
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
ZMYND21; SMYD4
Positive Sample:
MCF7,HeLa,Jurkat
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, nucleus
Calculated MW:
89kDa
Observed MW:
90kDa
Predicted to enable metal ion binding activity and methyltransferase activity. Involved in heart development. Predicted to be located in cytoplasm and nucleus.
Purification Method:
Affinity purification
Gene ID:
114826
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SMYD4 Rabbit pAb (CAB2337) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
