The SMPD1 Polyclonal Antibody (CAB6743) is specifically designed for research involving SMPD1, a gene encoding acid sphingomyelinase, an enzyme involved in the breakdown of sphingomyelin into ceramide and phosphorylcholine. The antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications such as Western blot and immunohistochemistry.SMPD1 plays a crucial role in lipid metabolism and cellular signaling pathways, making it a key target for studying lipid storage disorders and neurodegenerative diseases. By detecting and analyzing SMPD1 protein levels in different cell types and tissues, researchers can gain insights into the function and dysregulation of sphingolipid metabolism in various pathological conditions.
With its high specificity and sensitivity, the SMPD1 Polyclonal Antibody is an invaluable tool for investigators in the fields of biochemistry, molecular biology, and cell biology who are studying the role of sphingomyelinase in health and disease. Its versatility and reliability make it a valuable asset for advancing our understanding of lipid metabolism and developing targeted therapies for related disorders.
Product Name:
SMPD1/ASM Rabbit Polyclonal Antibody
SKU:
CAB6743
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1 / ASM (NP_000534.3).
The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
6609
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SMPD1 / ASM Rabbit pAb (CAB6743) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 5s.
