The SMPD1 Polyclonal Antibody (CAB16263) is specifically designed for researchers studying the sphingomyelin phosphodiesterase 1 (SMPD1) protein. This antibody, produced in rabbits, has high reactivity with human samples and is suitable for Western blot applications. SMPD1 is a key enzyme involved in sphingolipid metabolism and is essential for the breakdown of sphingomyelin into ceramide. Dysregulation of SMPD1 has been linked to various diseases, including Niemann-Pick disease and cancer.
By targeting SMPD1 with this antibody, researchers can investigate its role in cellular processes and disease pathogenesis.This antibody provides a valuable tool for studying SMPD1 expression and activity in different cell types, aiding in the understanding of sphingolipid metabolism and its implications in diseases. Researchers in fields such as biochemistry, cell biology, and oncology can benefit from using this antibody to further their research efforts.
Product Name:
SMPD1/ASM Rabbit Polyclonal Antibody
SKU:
CAB16263
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1 / ASM (NP_000534.3).
The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
6609
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SMPD1 / ASM antibody (CAB16263) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
