The SMG7 Polyclonal Antibody (CAB15371) is a crucial tool for researchers studying SMG7, a protein involved in RNA metabolism and mRNA degradation pathways. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for Western blot applications. By binding specifically to SMG7, researchers can accurately detect and analyze the protein in various cell types, making it an essential reagent for studies in molecular biology and RNA biology.SMG7 is a key player in the nonsense-mediated mRNA decay pathway, which serves as a quality control mechanism to eliminate aberrant mRNAs and regulate gene expression.
Dysregulation of this pathway has been linked to various human diseases, including neurodegenerative disorders and cancer. By studying SMG7 and its functions, researchers can gain a better understanding of mRNA surveillance mechanisms and potentially uncover new therapeutic targets for these conditions.Overall, the SMG7 Polyclonal Antibody is a valuable tool for researchers investigating RNA metabolism and mRNA degradation pathways, offering insight into the intricate processes that govern gene expression and potentially leading to the development of novel treatments for diseases associated with RNA dysregulation.
Product Name:
SMG7 Rabbit Polyclonal Antibody
SKU:
CAB15371
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMG7 (NP_963863.2).
This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
9887
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SMG7 Rabbit pAb (CAB15371) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
