The SMG5 Polyclonal Antibody (CAB18123) is a valuable tool for researchers studying SMG5, a protein involved in mRNA surveillance and nonsense-mediated mRNA decay. This rabbit polyclonal antibody specifically targets SMG5 in human samples and is recommended for use in Western blot applications. By binding to SMG5, researchers can study its expression levels and functions in various cell types, offering insights into RNA quality control mechanisms and potential therapeutic targets.
SMG5 is a key player in the mRNA surveillance pathway, where it helps to identify and degrade abnormal mRNA molecules to prevent the production of defective proteins. Dysregulation of this process has been linked to various diseases, including neurodegenerative disorders and cancer. By studying SMG5 using this antibody, researchers can uncover its role in maintaining cellular homeostasis and potentially identify new strategies for therapeutic intervention.
Product Name:
SMG5 Rabbit Polyclonal Antibody
SKU:
CAB18123
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 950-1016 of human SMG5 (NP_056142.2).
SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]).
Purification Method:
Affinity purification
Gene ID:
23381
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SMG5 antibody (CAB18123) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
