The SMARCA4 Polyclonal Antibody (CAB2117) is a high-quality antibody designed for researchers studying SMARCA4, a key protein involved in chromatin remodeling and gene expression regulation. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications.SMARCA4, also known as BRG1, is a crucial component of the SWItch/Sucrose Non-Fermentable (SWI/SNF) chromatin remodeling complex and is essential for normal development and cellular function. Dysregulation of SMARCA4 has been linked to a variety of diseases, including cancer and developmental disorders, making it an important target for research in molecular biology and oncology.
By specifically binding to the SMARCA4 protein, this antibody enables accurate detection and analysis of SMARCA4 in various cell types and tissues. Its versatility and reliability make it ideal for studies aimed at understanding the role of SMARCA4 in gene regulation, cellular differentiation, and disease pathways. Researchers can leverage the SMARCA4 Polyclonal Antibody to advance their investigations in cancer biology, epigenetics, and therapeutic development.
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6597
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and BRG1/BRG1/SMARCA4 knockout (KO) 293T cells, using [KO Validated] BRG1/SMARCA4 Rabbit pAb (CAB2117) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 1s.
