The Smad7 Polyclonal Antibody (CAB12343) is a valuable tool for researchers studying the Smad7 protein, a key regulator in the TGF-beta signaling pathway. This antibody is produced in rabbits and is specifically designed for use in Western blot applications. Smad7 is known for its role in inhibiting TGF-beta signaling, which is involved in various cellular processes including cell growth, differentiation, and immune responses. By targeting Smad7, researchers can gain insight into the mechanisms underlying these processes and potentially uncover new therapeutic targets for diseases such as cancer, fibrosis, and autoimmune disorders.
With its high reactivity to human samples, the Smad7 Polyclonal Antibody is ideal for studies in cell biology, immunology, and cancer research. Its ability to detect and analyze Smad7 protein levels in various cell types makes it a versatile tool for investigators looking to deepen their understanding of TGF-beta signaling and its impact on disease progression.
Product Name:
Smad7 Rabbit Polyclonal Antibody
SKU:
CAB12343
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 220-420 of human Smad7 (NP_005895.1).
The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4092
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Smad7 antibody (CAB12343) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
