The SMAD5 Polyclonal Antibody (CAB1947) is designed for research involving SMAD5, a key signaling molecule in the TGF-β pathway with crucial roles in cell growth, differentiation, and development. The antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It specifically binds to the SMAD5 protein, allowing for accurate detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and developmental biology.
SMAD5 is a critical component of the TGF-β signaling pathway, which plays a central role in various cellular processes such as cell proliferation, apoptosis, and differentiation. Dysregulation of SMAD5 signaling has been implicated in a wide range of diseases including cancer, fibrosis, and developmental disorders. Understanding the function of SMAD5 is essential for unraveling the complexities of these diseases and developing targeted therapies to treat them effectively.
Product Name:
Smad5 Rabbit Polyclonal Antibody
SKU:
CAB1947
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 186-465 of human Smad5 (NP_005894.3).
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4090
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat lung, using Smad5 Rabbit pAb (CAB1947) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
