The Smad5 Antibody (CAB1947) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It specifically binds to the SMAD5 protein, allowing for accurate detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and developmental biology.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Smad5 Antibody
SKU:
CAB1947
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DWFC, JV5-1, MADH5, Smad5
Positive Sample:
Rat lung
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
52kDa
Observed MW:
52kDa
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4090
RRID
AB_2763973
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using Smad5 Rabbit pAb (CAB1947) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human colon using Smad5 Rabbit pAb (CAB1947) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
