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Smad3 Transcription Factor Activity Assay

SKU:
TFAB00187
Product Type:
ELISA Kit
ELISA Type:
Transcription Factor Assay
Analytes:
Smad3
Reactivity:
Human
Mouse
Rat
$719
Frequently bought together:

Description

system_update_altDatasheet

Smad3 Transcription Factor Activity Assay

The SMAD3 Transcription Factor Activity Assay Kit is specifically designed for the precise measurement of SMAD3 transcription factor activity in cell lysates from various sample types. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications.SMAD3 is a key transcription factor involved in the TGF-β signaling pathway, regulating gene expression and playing a critical role in cell growth, differentiation, and apoptosis.

Dysregulation of SMAD3 activity has been linked to various diseases, including cancer, fibrosis, and autoimmune disorders, highlighting its importance as a potential therapeutic target and biomarker.With the SMAD3 Transcription Factor Activity Assay Kit, researchers can gain valuable insights into the molecular mechanisms governing cellular processes and disease pathogenesis, enabling them to advance their research and potentially develop novel treatments targeting SMAD3 signaling pathways.

Product Name:Smad3 Transcription Factor Activity Assay
Product Code:TFAB00187
Target:Smad3
Synonyms:JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3
Reactivity:Human, Mouse, Rat
Sample Types:Nuclear or cell lysates

The Assay Genie Smad3 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:4.5 hours
Detection Method:Colorimetric 450 nm
Size:12 x 8-Well Microstrips
Storage:4°C for 6 months
UniProt Protein Function:Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
NCBI Summary:The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
UniProt Code:P84022
NCBI GenInfo Identifier:51338669
NCBI Gene ID:4088
NCBI Accession:P84022.1
UniProt Secondary Accession:P84022,O09064, O09144, O14510, O35273, Q92940, Q93002 A8K4B6, B7Z4Z5, B7Z6M9, B7Z9Q2, F5H383,
UniProt Related Accession:P84022
Molecular Weight:25,722 Da
NCBI Full Name:Mothers against decapentaplegic homolog 3
NCBI Synonym Full Names:SMAD family member 3
NCBI Official Symbol:SMAD3  
NCBI Official Synonym Symbols:LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436  
NCBI Protein Information:mothers against decapentaplegic homolog 3
UniProt Protein Name:Mothers against decapentaplegic homolog 3
UniProt Synonym Protein Names:JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Protein Family:Mothers against decapentaplegic
UniProt Gene Name:SMAD3  
AntibodiesELISA Kits
Anti-Smad3 Antibody (CAB11388)Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) ELISA Kit (HUES02375)
Anti-Smad3 Antibody (CAB11471)Smad3 (Phospho-Thr179) Colorimetric Cell-Based ELISA Kit
Anti-Smad3 Antibody (CAB16913)Smad3 (Phospho-Ser213) Colorimetric Cell-Based ELISA Kit
Anti-Smad3 Antibody [KO Validated] (CAB19115)Smad3 (Phospho-Ser213) Transcription Factor Activity Assay
Anti-Phospho-SMAD3-T179 Antibody (CABP0554)

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