The Smad3 Polyclonal Antibody (CAB16913) is a valuable tool for research involving Smad3, a key transcription factor in the TGF-beta signaling pathway that plays a crucial role in cellular processes such as growth, differentiation, and inflammation. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for use in Western blot applications.Smad3 is a crucial mediator of TGF-beta signaling, regulating the expression of genes involved in cell proliferation, differentiation, and apoptosis. Dysregulation of the Smad3 pathway has been implicated in various diseases, including cancer, fibrosis, and immune disorders.
By targeting Smad3 with this antibody, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic strategies.In conclusion, the Smad3 Polyclonal Antibody is a valuable tool for studying the role of Smad3 in cellular processes and disease pathogenesis. Its high specificity and sensitivity make it an ideal choice for researchers in the fields of cell biology, immunology, and cancer research.
Product Name:
Smad3 Rabbit Polyclonal Antibody
SKU:
CAB16913
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human Smad3 (NP_005893.1).
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.
Purification Method:
Affinity purification
Gene ID:
4088
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using (CAB16913) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
