The SMAD3 Polyclonal Antibody (CAB11471) is a vital tool for researchers studying the SMAD3 protein, a key player in the TGF-β signaling pathway involved in cell growth, differentiation, and development. This antibody, generated in rabbits, offers high specificity and sensitivity for detecting SMAD3 in human samples, making it ideal for use in Western blot applications.SMAD3 is a critical mediator of various cellular processes, including proliferation, differentiation, and apoptosis, and dysregulation of its signaling pathway has been implicated in a range of diseases, including cancer, fibrosis, and cardiovascular disorders.
By targeting SMAD3, researchers can gain valuable insights into the mechanisms underlying these diseases and develop novel therapeutic strategies.With its superior performance in detecting SMAD3, the CAB11471 antibody is a valuable asset for scientists working in immunology, cancer biology, and developmental biology. Its ability to accurately detect and quantify SMAD3 levels in various cell types provides a powerful tool for investigating the role of this protein in both normal physiological processes and disease pathology.
Product Name:
Smad3 Rabbit Polyclonal Antibody
SKU:
CAB11471
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Smad3 (NP_005893.1).
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.
Purification Method:
Affinity purification
Gene ID:
4088
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of rat ovary, using Smad3 antibody (CAB11471) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
