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Smad3 (Phospho-Ser213) Transcription Factor Activity Assay

SKU:
TFAB00064
Product Type:
ELISA Kit
ELISA Type:
Transcription Factor Assay
Analytes:
Smad3 (Phospho-Ser213)
Reactivity:
Human
Mouse
Rat
€599
Frequently bought together:

Description

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Smad3 (Phospho-Ser213)Transcription Factor Activity Assay

The SMAD3 (Phospho-Ser213) Transcription Factor Activity Assay is a cutting-edge kit designed for the accurate and specific detection of SMAD3 activity in cellular samples. By targeting the phosphorylation of Ser213 on SMAD3, this assay allows researchers to analyze the transcriptional activity of this key signaling molecule.SMAD3 is a critical regulator of TGF-β signaling, playing a pivotal role in cell growth, differentiation, and development. Dysregulation of SMAD3 activity has been linked to various diseases, including cancer, fibrosis, and inflammatory disorders, making it an attractive target for therapeutic intervention.

With its high sensitivity and precision, the SMAD3 (Phospho-Ser213) Transcription Factor Activity Assay offers a reliable tool for studying SMAD3 function in both basic research and drug discovery. Whether investigating cellular pathways or developing novel therapies, this assay provides valuable insights into the role of SMAD3 in health and disease.

Product Name:Smad3 (Phospho-Ser213) Transcription Factor Activity Assay
Product Code:TFAB00064
Target:Smad3 (Phospho-Ser213)
Synonyms:JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3
Reactivity:Human, Mouse, Rat
Sample Types:Nuclear or cell lysates

The Assay Genie Smad3 (Phospho-Ser213) transcription factor activity assay allows for the detection and qualitative analysis of Smad3 (Phospho-Ser213) active transcription factors in eukaryotic nuclear or cell lysates.

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:4.5 hours
Detection Method:Colorimetric 450 nm
Size:12 x 8-Well Microstrips
Storage:4°C for 6 months
UniProt Protein Function:Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
NCBI Summary:The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
UniProt Code:P84022
NCBI GenInfo Identifier:51338669
NCBI Gene ID:4088
NCBI Accession:P84022.1
UniProt Secondary Accession:P84022,O09064, O09144, O14510, O35273, Q92940, Q93002 A8K4B6, B7Z4Z5, B7Z6M9, B7Z9Q2, F5H383,
UniProt Related Accession:P84022
Molecular Weight:25,722 Da
NCBI Full Name:Mothers against decapentaplegic homolog 3
NCBI Synonym Full Names:SMAD family member 3
NCBI Official Symbol:SMAD3  
NCBI Official Synonym Symbols:LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436  
NCBI Protein Information:mothers against decapentaplegic homolog 3
UniProt Protein Name:Mothers against decapentaplegic homolog 3
UniProt Synonym Protein Names:JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Protein Family:Mothers against decapentaplegic
UniProt Gene Name:SMAD3  
AntibodiesELISA Kits
Anti-Smad3 Antibody (CAB11388)Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) ELISA Kit (HUES02375)
Anti-Smad3 Antibody (CAB11471)Smad3 (Phospho-Thr179) Colorimetric Cell-Based ELISA Kit
Anti-Smad3 Antibody (CAB16913)Smad3 (Phospho-Ser213) Colorimetric Cell-Based ELISA Kit
Anti-Smad3 Antibody [KO Validated] (CAB19115)Smad3 Transcription Factor Activity Assay
Anti-Phospho-SMAD3-T179 Antibody (CABP0554)

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