The SMAD2 Polyclonal Antibody (CAB16912) is a valuable tool for researchers studying SMAD2, a critical component of the TGF-beta signaling pathway. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it an ideal choice for various research applications, including Western blotting.SMAD2 is a key mediator of TGF-beta signaling, playing a crucial role in cellular processes such as growth, differentiation, and apoptosis. Dysregulation of the TGF-beta pathway, including SMAD2, has been implicated in various diseases, including cancer, fibrosis, and developmental disorders.
The SMAD2 Polyclonal Antibody enables researchers to detect and analyze SMAD2 protein expression in different cell types, providing valuable insights into its function and potential therapeutic targets.By using the SMAD2 Polyclonal Antibody, researchers can further understand the role of SMAD2 in disease pathogenesis and identify novel strategies for therapeutic intervention. Its high specificity and reactivity make it a reliable tool for investigations in the fields of cancer biology, developmental biology, and signal transduction pathways.
Product Name:
Smad2 Rabbit Polyclonal Antibody
SKU:
CAB16912
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 175-270 of human Smad2 (NP_005892.1).
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
4087
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-1080 cells, using Smad2 Rabbit pAb (CAB16912) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
