The Smad2 Polyclonal Antibody (CAB11498) is a valuable tool for researchers studying the Smad2 protein, which plays a key role in the TGF-β signaling pathway. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the Smad2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for research in the fields of cell biology, developmental biology, and cancer research.
Smad2 is a signal transducer that mediates the effects of TGF-β signaling, regulating processes such as cell growth, differentiation, and apoptosis. Dysregulation of the TGF-β signaling pathway, including abnormalities in Smad2 activity, has been implicated in various diseases, including cancer, fibrosis, and developmental disorders. By studying the function of Smad2, researchers can gain valuable insights into these diseases and potentially identify new therapeutic targets for intervention.
Product Name:
Smad2 Rabbit Polyclonal Antibody
SKU:
CAB11498
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad2 (NP_005892.1).
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
4087
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Smad2 Rabbit pAb (CAB11498) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
