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Smad2 Rabbit Polyclonal Antibody (CAB11498)

SKU:
CAB11498
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-Smad2 Antibody CAB11498
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-Smad2 Antibody CAB11498
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-Smad2 Antibody CAB11498
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Smad2 Rabbit Polyclonal Antibody
SKU:CAB11498
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad2 (NP_005892.1).
Sequence:MSSI LPFT PPVV KRLL GWKK SAGG SGGA GGGE QNGQ EEKW CEKA VKSL VKKL KKTG RLDE LEKA ITTQ NCNT KCVT IPST CSEI WGLS TPNT IDQW DTTG
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:JV18; LDS6; CHTD8; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; Smad2
Positive Sample:HT-1080,C2C12,C6,Rat testis
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:52kDa
Observed MW:60kDa

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.

Purification Method:Affinity purification
Gene ID:4087
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Smad2 Rabbit pAb (CAB11498) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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